Document Type
Original Study
Subject Areas
Pharmaceutical Analytical Chemistry
Keywords
Micellar liquid chromatography, Green micellar method, Azilsartan medoxomil, chlorthalidone, Flow-gradient, validation
Abstract
A green novel cost effective flow-gradient micellar high performance liquid chromatographic method was developed and validated for the simultaneous determination of two antihypertensive drugs,Azilsartan Medoxomil (AZL) and chlorthalidone (CLT) in plasma and urine. The eco-friendly method showed good linearity over the ranges of 15.00-80.00 µg/mL and 12.50-62.50 µg/mL with limits of detection (LOD) of 0.19, 0.12 µg/mL and limits of quantification (LOQ) of 0.59, 0.36 µg/mL for AZL and CLT, respectively. The proposed method was successfully applied for the simultaneous analysis of the studied drugs in their single tablets, laboratory mixed tablets in human plasma and urine without prior extraction procedure. The method was verified on the Analytical Greenness metric approach (AGREE) and it was found to be an excellent green method.
How to Cite This Article
El-Alamin, Maha M. Abou; Sultan, Maha A.; Mohamed, Dina A; and Azab, Marwa M.
(2024)
"Green Flow-gradient Micellar High Performance Liquid Chromatograpic method for Determination of a Hypertensive Combination ; Azilsartan Medoxomil and Chlorthalidone :Application to Spiked Human Plasma and Urine,"
Trends in advanced sciences and technology: Vol. 1, Article 15.
DOI: 10.62537/2974-444X.1016
Available at:
https://tast.researchcommons.org/journal/vol1/iss1/15
